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Equine Reproductive Procedures

Equine Reproductive Procedures

9781119555988
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Description

The second edition of Equine Reproductive Procedures provides equine practitioners, veterinary students, and equine theriogenologists with a fully updated, practical guide to techniques in equine reproductive practice. This easy-to-use resource covers skills-based information with a clinical focus, taking an easy-to-follow step-by-step approach.

The book covers topics related to the reproductive management of horses, including diagnostic techniques and therapeutic procedures for stallions, mares, and foals. Procedures highlighted in the book are supported with clear descriptions and photographs. Readers will also find a list of required supplies for the procedure and a reference list.

  • Provides step-by-step descriptions of techniques related to equine theriogenology
  • Serves as a valuable practice tool
  • Offers 39 new techniques not found in the first edition
  • Includes key updates to existing techniques

 Those studying equine reproduction and specialists in theriogenology will find this an essential ‘how-to’ guide for their practice library.

Product Details
101419
9781119555988

Data sheet

Publication date
2021
Issue number
2
Cover
hard cover
Pages count
784
Dimensions (mm)
224.00 x 280.00
  • List of Contributors xiii

    Preface xv

    Part I Non-Pregnant Mare 1

    1 Reproductive Evaluation 3
    Patrick M. McCue

    2 Teasing 7
    Patrick M. McCue

    3 Tail Wrap and Preparation/Washing of the Perineum 11
    John J. Dascanio

    4 Placement of a Tail Rope 17
    John J. Dascanio

    5 Perineal Conformation Evaluation 21
    John J. Dascanio

    6 Removal of a Persistent Hymen 25
    John J. Dascanio

    7 Palpation of the Reproductive Tract 27
    Patrick M. McCue

    8 Ultrasound Evaluation 31
    Patrick M. McCue

    9 Prediction of Ovulation 37
    Patrick M. McCue

    10 Speculum Examination of the Vagina 41
    John J. Dascanio

    11 Digital Examination of the Vagina/Cervix 45
    Sofie Sitters

    12 Uterine Culture Collection: Swab/Brush 49
    John J. Dascanio

    13 Antimicrobiotic Sensitivity Testing 53
    Jillian Bishop and Patrick M. McCue

    14 Microbiology: Microbial Culture 5
    Patrick M. McCue and Jillian Bishop

    15 Microbiology: Gram Stain 59
    Jillian Bishop and Patrick M. McCue

    16 qPCR Assay for the Diagnosis of Infectious Endometritis 61
    Ryan A. Ferris and Patrick M. McCue

    17 Uterine Cytology Collection: Swab/Brush 67
    John J. Dascanio and Ryan A. Ferris

    18 Uterine Culture/Cytology: Low Volume Lavage 73
    John J. Dascanio

    19 Endometrial Biopsy 77
    Patrick M. McCue

    20 Hysteroscopic Examination of the Uterus 81
    Patrick M. McCue

    21 Endometrial Cyst Removal 85
    John J. Dascanio

    22 Chromosomal Analysis 89
    Terje Raudsepp

    23 Endocrinological Examination 97
    Patrick M. McCue

    24 Laparoscopic Examination of the Female Reproductive Track with Ovarian Biopsy 99
    Dean A. Hendrickson

    25 Evaluation of the Mammary Gland 103
    Patrick M. McCue

    26 Antisperm Antibody Testing 107
    Patrick M. McCue

    27 Starch Granule Test for the Evaluation of Oviductal Patency 109
    Sofie Sitters and John J. Dascanio

    28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency 111
    Sofie Sitters and John J. Dascanio

    29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency 113
    Sofie Sitters and Patrick M. McCue

    30 Prostaglandin E2 Treatment for Blocked Oviducts 117
    Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

    31 Prostaglandin E1 Treatment for Blocked Oviducts 119
    Patrick M. McCue and Charles F. Scoggin

    32 Hysteroscopic Hydrotubation of the Oviducts 121
    Charles F. Scoggin

    33 Transvaginal Ovarian Biopsy 123
    Claire E. Card

    34 Uterine Lavage 127
    John J. Dascanio

    35 Uterine Infusion 131
    John J. Dascanio

    36 Therapeutics for Infectious Endometritis 133
    Ryan A. Ferris

    37 Bacterial Biofilm and Endometritis 137
    Ryan A. Ferris

    38 Latent Uterine Bacterial Infections 141
    Morten Ronn Petersen and Anders Miki Bojesen

    39 Acupuncture in Mare Reproduction 143
    Kristina Lu and Lauren Javernick

    40 Restraint for Breeding 149
    John J. Dascanio

    41 Natural Service: Pasture Breeding 151
    Patrick M. McCue

    42 Natural Service: In-hand Breeding 153
    Charles F. Scoggin

    43 Breeding Stitches 157
    Charles F. Scoggin

    44 Reinforcement Breeding 159
    Charles F. Scoggin

    45 Breeding with Fresh or Cooled Semen 161
    John J. Dascanio and Patrick M. McCue

    46 Insemination Through a Vaginal Speculum 165
    Patrick M. McCue

    47 Breeding with Frozen Semen 167
    John J. Dascanio

    48 Deep Horn Insemination 171
    John J. Dascanio

    49 Hysteroscopic (Low Dose) Insemination 173
    Patrick M. McCue

    50 Transvaginal Oocyte Pickup 177
    Jennifer N. Hatzel

    51 Packaging and transport of oocytes 183
    Jennifer N. Hatzel

    52 Intracytoplasmic Sperm Injection 187
    Jennifer N. Hatzel and JoAnne Stokes

    53 Caslick Operation or Vulvoplasty 191
    John J. Dascanio

    54 Contagious Equine Metritis Testing 195
    John J. Dascanio

    55 Management of Seasonal Anestrus: Artificial Photoperiod 199
    Patrick M. McCue

    56 Management of Seasonal Anestrus: Hormone Therapy 201
    Patrick M. McCue

    57 Hormone Therapy in Cycling Mares 205
    Patrick M. McCue

    58 Estrus Suppression 211
    Patrick M. McCue

    59 Intrauterine Devices for Estrus Suppression 215
    Carlos M. Gradil

    60 Immunocontraception 221
    Patrick M. McCue

    61 Harvesting and Shipping Ovaries for Oocyte Recovery 223
    Patrick M. McCue

    Part II Pregnant Mare 225

    62 Embryo Collection 227
    Patrick M. McCue

    63 Embryo Evaluation 231
    Patrick M. McCue

    64 Embryo Biopsy 235
    Patrick M. McCue

    65 Embryo Packaging for Cooled Transport 237
    Patrick M. McCue

    66 Embryo Cryopreservation 241
    Patrick M. McCue

    67 Non-Surgical Embryo Transfer 245
    Patrick M. McCue

    68 Autogenous Transfer of ICSI-Derived Embryos 249
    Jennifer N. Hatzel

    69 Palpation per Rectum of the Pregnant Reproductive Tract 253
    Sofie Sitters

    70 Ultrasound Examination of the Pregnant Reproductive Tract 257
    Patrick M. McCue

    71 Speculum Examination of the Pregnant Mare 263
    Sofie Sitters

    72 Determination of Fetal Sex at 55–200 days 265
    Richard D. Holder

    73 Assessment of Late-Term Fetal Well-being 273
    John J. Dascanio

    74 Combined Thickness of the Uterus and Placenta 277
    Patrick M. McCue

    75 Placentitis: Diagnosis and Treatment 281
    Justin W. McNaughten and Margo L. Macpherson

    76 Doppler Ultrasonography 287
    John J. Dascanio

    77 Prediction of Fetal Age 291
    John J. Dascanio

    78 Twin Reduction: Manual Technique 295
    John J. Dascanio

    79 Twin Reduction: Transvaginal Aspiration 299
    John J. Dascanio

    80 Twin Reduction: Cranio-Cervical Dislocation 303
    Karen Wolfsdorf

    81 Twin Reduction: Thoracic Compression 307
    Patrick M. McCue

    82 Twin Reduction: Transabdominal Fetal Cardiac Puncture 309
    John J. Dascanio

    83 Elective Termination of Pregnancy 313
    John J. Dascanio

    84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission 317
    Julie T. Cecere and John J. Dascanio

    85 Endocrine Evaluation of Pregnancy 319
    Patrick M. McCue

    86 Treatment of Vaginal Varicosities 323
    John J. Dascanio

    87 Manual Correction of Uterine Torsion 327
    John J. Dascanio

    88 Preparation for Foaling 331
    John J. Dascanio

    89 Assessment of Mammary Gland Secretions to Predict Foaling 335
    John J. Dascanio

    90 Assessment of pH of Mammary Gland Secretions to Predict Foaling 339
    Patrick M. McCue

    91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras 343
    Ryan A. Ferris

    92 Foaling Alert: Vulvar Device 345
    John J. Dascanio

    93 Positional Labor Alert Devices 347
    Ryan A. Ferris

    94 Induction of Parturition 351
    John J. Dascanio

    95 Normal Foaling 355
    John J. Dascanio

    96 Premature Separation of the Placenta 361
    Patrick M. McCue

    97 Dystocia Management 365
    Patrick M. McCue and Sofie Sitters

    98 Dystocia Correction 369
    John J. Dascanio

    99 Fetotomy 375
    John J. Dascanio

    100 Epidural 381
    John J. Dascanio

    101 Induction of Lactation to Create a Nurse Mare 383
    Pouya Dini and Peter F. Daels

    102 Screening to Prevent Neonatal Isoerythrolysis 385
    Patrick M. McCue

    Part III Postpartum Mare 387

    103 Evaluation of Colostrum Specific Gravity 389
    Patrick M. McCue

    104 Evaluation of Colostrum Quality: Brix Refractometry 393
    Patrick M. McCue

    105 Colostrum Banking 395
    Patrick M. McCue

    106 Obtaining Milk from the Mare 399
    John J. Dascanio

    107 Placental Evaluation 401
    Patrick M. McCue

    108 Removal of Retained Placenta 407
    John J. Dascanio

    109 Abdominocentesis 413
    John J. Dascanio

    110 Uterine Prolapse Treatment 415
    John J. Dascanio

    111 Buhner Needle Placement of a Perivulvar Suture 419
    John J. Dascanio

    Part IV Stallion 423

    112 Breeding Soundness Evaluation 425
    Patrick M. McCue

    113 Training the Stallion to Use a Phantom 429
    John J. Dascanio

    114 Missouri Artificial Vagina 433
    John J. Dascanio and Lynda M. J. Miller

    115 Colorado Model Artificial Vagina 437
    Patrick M. McCue

    116 Roanoke Artificial Vagina 441
    John J. Dascanio

    117 Hannover Artificial Vagina 445
    Sofie Sitters

    118 Teaser Stallions 447
    Charles F. Scoggin

    119 Standing Semen Collection 449
    John J. Dascanio

    120 Collection of Semen Using an Open-Ended Artificial Vagina 453
    Patrick M. McCue

    121 Chemical Ejaculation 455
    Patrick M. McCue

    122 Preparation of a Jump Mare or a Mare for Natural Cover 457
    John J. Dascanio

    123 Washing the Penis 459
    John J. Dascanio

    124 Evaluation of Sexual Behavior 463
    John J. Dascanio and Sue M. McDonnell

    125 Enhancement of Sexual Interest and Response 467
    Sue M. McDonnell

    126 Management of Ejaculation Difficulty 471
    Sue M. McDonnell

    127 Calibrated Spectrophotometer Evaluation of Sperm Concentration 475
    John J. Dascanio

    128 Hemocytometer Evaluation of Sperm Concentration 479
    John J. Dascanio

    129 NucleoCounter® Evaluation of Sperm Concentration and Viability 483
    Patrick M. McCue

    130 Visual Evaluation of Sperm Motility 489
    Patrick M. McCue

    131 Computer-Assisted Sperm Analysis 491
    John J. Dascanio and Lynda M. J. Miller

    132 Eosin-Nigrosin Staining in the Evaluation of Sperm 495
    Julie T. Cecere

    133 Evaluation of Sperm Morphology 499
    Leonardo Brito

    134 Determination of Daily Sperm Output 505
    Patrick M. McCue

    135 Measurement of Testicular Size and Estimation of Daily Sperm Output 507
    Patrick M. McCue

    136 Wet Mount Evaluation of Sperm 511
    John J. Dascanio

    137 Diff-Quik® Evaluation of Round Cells and Sperm 513
    John J. Dascanio and Lynda M. J. Miller

    138 Electron Microscopy of Constituents of Seminal Ejaculates 515
    D. N. Rao Veeramachaneni

    139 Bacterial Culture 519
    John J. Dascanio

    140 Evaluation of pH and Osmolarity of Semen 521
    Patrick M. McCue

    141 Alkaline Phosphatase: A Marker for Ejaculation 523
    Patrick M. McCue

    142 Diagnosis and Management of Urospermia 525
    John J. Dascanio

    143 Hemospermia 529
    Patrick M. McCue

    144 Semen Extenders and Sperm Media 533
    Patrick M. McCue

    145 Preparation of Semen for Cooled Transport 537
    Etta A. Bradecamp

    146 Packing Semen for Cooled Transport 541
    Etta A. Bradecamp

    147 Longevity Testing of Sperm 547
    John J. Dascanio

    148 Calculation of g Force for Centrifuging Semen 549
    John J. Dascanio

    149 Centrifugation of Semen: Standard Technique 551
    Etta A. Bradecamp

    150 Centrifugation of Semen: Cushion Technique 557
    Etta A. Bradecamp

    151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique 561
    Etta A. Bradecamp

    152 Use of a SpermFilter 563
    Marco A. Alvarenga and Lorenzo G. T. M. Segabinazzi

    153 Semen Freezing 567
    Patrick M. McCue

    154 Refreezing Semen 573
    Patrick M. McCue

    155 Thawing Frozen Semen 577
    John J. Dascanio

    156 Semen Processing for Intracytoplasmic Sperm Injection 581
    Patrick M. McCue

    157 Preparing a Vapor Shipper 585
    Julie T. Cecere and Paul Loomis

    158 Loading a Vapor Shipper 589
    Julie T. Cecere and Paul Loomis

    159 Maintaining a Long-Term Frozen Semen Storage Tank 591
    John J. Dascanio

    160 International Transport of Frozen Semen 593
    Paul Loomis

    161 Harvesting and Shipping Testes 597
    John J. Dascanio

    162 Epididymal Sperm Recovery 599
    John J. Dascanio and Lynda M. J. Miller

    163 Endoscopic Examination of the Urethra 603
    Patrick M. McCue

    164 Endoscopic-Guided Cannulation of the Seminal Vesicles 607
    Patrick M. McCue

    165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord 609
    Sofie Sitters

    166 Palpation and Ultrasonography of the Accessory Sex Glands 615
    Sofie Sitters

    167 Radiographic Examination of the Penis 621
    Patrick M. McCue

    168 Testicular Biopsy and Aspiration 623
    John J. Dascanio

    169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14 627
    Amanda I. Glazar, and Patrick M. McCue

    170 Sperm Chromatin Structure Assay 629
    Charles Love

    171 Hypo-Osmotic Swelling Test 633
    Patrick M. McCue

    172 Assessment of Sperm Acrosomal Status: PE-PNA 635
    Amanda I. Glazar and Patrick M. McCue

    173 Assessment of Sperm Mitochondrial Function: JC–1 and Rhodamine 123 637
    Amanda I. Glazar, and Patrick M. McCue

    174 Equine Viral Arteritis Testing 639
    G. Reed Holyoak and Udeni B. R. Balasuriya

    175 Diagnostic Endocrinology: Baseline Hormone Levels 645
    Patrick M. McCue

    176 Diagnostic Endocrinology: GnRH Stimulation Tests 647
    Patrick M. McCue

    177 Diagnostic Endocrinology: hCG Stimulation Test 649
    Patrick M. McCue

    178 Diagnostic Endocrinology: Estrogen Conjugate Assay 653
    Patrick M. McCue

    179 Diagnostic Tests for Cryptorchidism 655
    Patrick M. McCue

    180 Probang Apparatus 659
    Julie T. Cecere

    181 Support Apparatus for Paraphimosis 663
    John J. Dascanio

    182 Equine Castration Techniques 667
    Stacy Anderson

    183 Stallion Acupuncture 671
    Rhonda A. Rathgeber

    Part V Newborn Foal 675

    184 Birth Resuscitation 677
    Patrick M. McCue

    185 Handling and Restraint 681
    Undine Christmann

    186 Madigan Foal Squeeze Technique 687
    John Madigan

    187 Evaluation of Passive Transfer 691
    Patrick M. McCue

    188 Colostral Administration via Naso-Gastric Intubation 695
    John J. Dascanio

    189 Sedation 699
    Undine Christmann

    190 Intravenous Catheter Placement 701
    Undine Christmann

    191 Plasma Therapy in the Foal 707
    Undine Christmann

    192 Acupuncture in the Neonate 711
    Peter Morresey

    193 Routine Care 713
    Patrick M. McCue

    194 Foal Rejection 719
    Patrick M. McCue

    195 Fostering a Foal onto a Nurse Mare 723
    Peter F. Daels and Pouya Dini

    196 Entropion 725
    John J. Dascanio

    197 Umbilical Ultrasound 727
    Undine Christmann

    198 Management of Soft Tissue Laxity 733
    Stacy Anderson

    199 Management of Angular Limb Deformities 735
    Stacy Anderson

    200 Management of Congenital Flexural Limb Deformities 739
    Stacy Anderson

    Part VI Appendices 745

    Appendix A Society for Theriogenology Stallion Reproductive Evaluation Form 745

    Appendix B Mare Breeding Soundness Evaluation Form 749

    Appendix C Formulary for Equine Reproduction 751

    Appendix D Foaling Kit and Associated Equipment and Supplies 759

    Index 761

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