Phage Display

A Practical Approach

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Brand: OUP Oxford

ISBN: 9780199638734

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Description

This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date,comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, or wishing to use the technique for basic research and drug discovery.

Product Details

Brand OUP Oxford

Reference 84890

EAN13 9780199638734

ISBN 9780199638734

Data sheet

Publication date: 2004
Issue number: 1
Cover: paperback
Pages count: 356
Dimensions (mm): 189 x 246
Weight (g): 715

Introduction to phage biology and phage display; Constructing phage display libraries by oligonucleotide-directed mutagenesis; In vitro DNA recombination; Phage selection strategies for improved affinity and specificity of proteins and peptides; Rapid screening of phage displayed protein binding affinities by phage ELISA; Identification of novel ligands for receptors using recombinant peptide libraries; Substrate phage display; Protease-based selection of stably folded proteins and protein domains from phage display libraries; Phage display of zinc fingers and other nucleic acid-binding motifs; In vivo and ex vivo selections using phage-displayed libraries; Screening phage libraries with sera; Interaction cloning using cDNA libraries displayed on phage; Phage antibody libraries; Affinity maturation of phage antibodies;