The measurement of metabolic rates is central to important questions in many areas of physiological research. Unfortunately these measurements are anything but straightforward, with numerous pitfalls awaiting both the novice and even the experienced investigator. The original edition of this work, published in 2008, quickly became the principle how to manual for the field. It successfully de-mystified the topic, explaining every common variation of metabolic rate measurement. Background information on different analyzer and equipment types allowed users to choose the best instruments for their application. Respirometry equations, normally a topic of terror and confusion to researchers, were derived and described in sufficient detail to facilitatetheir selection and use. In this new edition, the content has been thoroughly updated and a decade of new literature incorporated. New chapters on room calorimetry, human metabolic measurement, and metabolic phenotyping have also been added.Measuring Metabolic Rates is aimed at experimental biologists, physiologists, and any professional scientist involved with metabolic measurement. This practical handbook will also be of relevance and use to graduate students.
Measuring the fire of life: a brief history of metabolic measurement; Constant volume and constant pressure respirometry; Coulometric respirometry; Constant volume techniques using gas analysis; Aquatic oxygen analysis; Direct calorimetry; Measuring field metabolic rates; Flow-through respirometry: the basics; Flow-through respirometry: the equations; Flow-through respirometry: incurrent flow measurement; Flow-through respirometry: excurrent flow measurement; Room calorimetry and mask respirometry; Metabolic phenotyping; Validating flow-through respirometry; Data analysis and presentation; The varieties of gas analyzers; The varieties of flow meters; The varieties of activity detectors; The varieties of scrubbers, tubing, and tubing connectors; Acquiring useful tools and skills;
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