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Manual of Immunopathological Techniques

Manual of Immunopathological Techniques

9789351520849
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Description

The use of immunopathological techniques has become increasingly widespread due to their ability to not only diagnose infectious and immunodeficiency disorders, but also haematological, oncological and gastroenterological diseases.

This manual is a practical guide to immunopathological techniques for undergraduates and trainees. Beginning with an overview of immunopathology, the following chapters provide in depth discussion on different techniques for the diagnosis and management of numerous immunological disorders, including autoimmune diseases, tumours, transplants and immunohistochemistry.

Presented in bullet point format for easy reference, this useful text is enhanced by high quality photographs and tables to assist learning.

Key points

  • Practical guide to immunopathological techniques
  • Covers different techniques for diagnosis and treatment of numerous disorders
  • Presented in bullet point format for easy reference
  • Highly illustrated with clinical photographs and tables
Product Details
JP Medical Publishers
95465
9789351520849
9789351520849

Data sheet

Publication date
2014
Issue number
1
Cover
paperback
Pages count
180
Dimensions (mm)
171.00 x 241.00
Weight (g)
290
    1. Introduction
    2. Tests for cell mediated immunity (CMI)
    3. Lymphocytes typing by Flow Cytometry
    4. Diagnosis of Kala-Azar by PCR technique
    5. Test for myeloma profile and qualitative assessment of serum proteins
    6. Agarose gel electrophoresis for qualitative assessment of serum proteins
    7. Cellulose Acetate Electrophoresis
    8. Agar Gel Immunoelectrophoresis for typing of myeloma
    9. Micro Agar Gel Double Diffusion Technique
    10. Counter Current Immunoelectrophoresis
    11. Single Radial Immunodiffusion and Immunoturbidometric methods for the estimation of Immunoglobulins
    12. Estimation of complement C3, C4 and hemolytic assay for serum complement
    13. Demonstration of immune complexes and complement by indirect immuno peroxidase method
    14. Test for autoimmune diseases
    15. Test for Autoantibodies by ELISA technique
    16. Acid Fuchsin Orange G in kidney biopsy for demonstration of immune complexes
    17. Detection of tumor markers in paraffin embedded sections by immunohistochemistry
    18. Detection of circulating immune complexes (CIC) in the serum
    19. Demonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescence
    20. HLA typing and Cross Match test in solid organ transplantation
    21. HLA B27 typing by PCR method
    22. Fluorescence in-situ hybridisation:: methods and applications
    23. Detection of cytokines (IL-6, IL-8, TNF-a)
    24. Western Blot or Tests for detection of antibody to extractable nuclear antigen
    25. Detection of anti-thyroglobulin antibodies by hemagglutination method
    26. Distillation and demineralization of water
    27. Raising of polyclonal anti human anti-IgG in rabbit
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