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Single-Molecule Science: From Super-Resolution Microscopy to DNA Mapping and Diagnostics

Single-Molecule Science: From Super-Resolution Microscopy to DNA Mapping and Diagnostics

9781108423366
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Description
Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology.
Product Details
93619
9781108423366
9781108423366

Data sheet

Publication date
2022
Issue number
1
Cover
hard cover
Pages count
550
Dimensions (mm)
174.00 x 250.00
Weight (g)
500
  • Preface; I. Super Resolution Microscopy and Molecular Imaging Techniques to Probe Biology:: 1. Introduction on Single Molecule Science Krishnarao Appasani and Raghu K. Appasani; 2. One molecule, two molecules, red molecules, blue molecules:: Methods for quantifying localization microscopy data George Patterson and Gaetan G. Herbomel; 3. Muti-scale fluorescence imaging Ruben Bulkescher, Inn Chung, Jan Philipp Eberle, Holger Erfle, Manuel Gunkel, Jürgen Reymann, Karsten Rippe, Guido Sauter, Ronald Simon and Vytaute Starkuviene; 4. Long-read single-molecule optical maps Assaf Grunwald, Yael Michaeli and Yuval Ebenstein; II. Protein Folding, Structure, Confirmation and Dynamics:: 5. Single-molecule mechanics of protein nanomachines Gabriel Žoldak and Katarzyna Tych; 6. Post-translational Protein Translocation Through Membranes at the Single Molecule Level Diego Quiroga-Roger, Hilda M. Alfaro-Valdes and Christian A.M. Wilson Moya; III. Mapping DNA Molecules at Single Molecule Level:: 7. Observing Dynamic states of single molecule DNA and Proteins using Atomic Force Microscope Jingqiang Li, Sithara Wijeratne and Ching-Hwa Kiang; 8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification Sourav Mishra, Yoonhee Lee and Joon Won Park; IV. Single Molecule Biology to Study Gene Expression:: 9. Single molecule detection in the study of gene expression Vipin Kumar, Simon Leclerc and Yuichi Taniguchi.
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