The fourth edition of this popular textbook retains its focus on the fundamental principles of gene manipulation, providing an accessible and broad-based introduction to the subject for beginning undergraduate students. It has been brought thoroughly up to date with new chapters on the story of DNA and genome editing, and new sections on bioethics, significant developments in sequencing technology and structural, functional and comparative genomics and proteomics, and the impact of transgenic plants. In addition to chapter summaries, learning objectives, concept maps, glossary and key word lists the book now also features new concluding sections, further reading lists and web-search activities for each chapter to provide a comprehensive suite of learning resources to help students develop a flexible and critical approach to the study of genetic engineering.
Preface; Part I. Genetic Engineering in Context: 1. Introduction; 2. The story of DNA; 3. Brave new world or genetic nightmare?; Part II. The Basis of Genetic Engineering: 4. Introducing molecular biology; 5. The tools of the trade; 6. Working with nucleic acids; Part III. The Methodology of Gene Manipulation: 7. Host cells and vectors; 8. Cloning strategies; 9. The polymerase chain reaction; 10. Selection, screening and analysis of recombinants; 11. Bioinformatics; 12. Genome editing; Part IV. Genetic Engineering in Action: 13. Investigating genes, genomes and otheromes; 14. Genetic engineering and biotechnology; 15. Medical and forensic applications of gene manipulation; 16. Transgenic plants and animals; 17. The other sort of cloning; Glossary; Index.
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