This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes:: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather tostimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.
Introduction to DNA; Manipulating DNA; Fluorescence Spectroscopy and Microscopy Techniques; The Mechanical Properties of Nucleic Acids; Structural Transitions in DNA; Single Molecule Studies of DNA-associated Motors; DNA and RNA Polymerases; DNA Helicases; Topoisomerases; Conclusions;
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