The authors and publisher are pleased to present the twenty-seventh edition of Harper’s Illustrated Biochemistry. First published as Review of Physiological Chemistry in 1939 and revised in 1944, it quickly gained a wide readership. In 1951, the third edition appeared with Harold A. Harper, University of California at San Francisco, as author. Dr. Harper remained the sole author until the ninth edition and co-authored eight subsequent editions. Peter Mayes and Victor Rodwell joined as authors in the tenth edition, Daryl Granner in the twentieth, and Rob Murray in the twenty-first edition. We now bid a fond and grateful farewell to our long-time colleague Peter Mayes, who retired from active authorship after the previous edition. Asked by Harold Harper to review portions of the ninth edition, Peter joined as an author for the tenth through twenty-sixth editions. Peter contributed a unique ability to design diagrams that integrate all key aspects of a metabolic pathway:: the enzymes, intermediates, and the mechanisms that guide and regulate metabolic flow. Such skills, his concise and informative prose, and his collegial relationships with the other authors contributed significantly to the continued success of this text. He will be greatly missed by his fellow authors and by readers. The ever-increasing complexity of biochemical knowledge has led to the addition of several co-authors in recent editions. Peter Mayes’ responsibilities now pass to his former collaborators, David Bender and Kathleen Botham, continuing the long-standing trans-Atlantic ties in the authorship of this text. P. Anthony Weil, a co-author with Daryl Granner in the previous edition, continues his invaluable input. Additional co-authors for this and prior editions include Fred Keeley and Margaret Rand with Rob Murray and Peter Kennelly with Victor Rodwell. The senior authors are grateful to their new colleagues for bringing their expertise and fresh perspectives to the text.
SECTION I. STRUCTURES & FUNCTIONS OF PROTEINS & ENZYMES 3. Amino Acids & Peptides 4. Proteins:: Determination of Primary Structure 5. Proteins:: Higher Orders of Structure 6. Proteins:: Myoglobin & Hemoglobin 7. Enzymes:: Mechanism of Action 8. Enzymes:: Kinetics 9. Enzymes:: Regulation of Activities 10. Bioinformatics & Computational Biology
SECTION II. BIOENERGETICS & THE METABOLISM OF CARBOHYDRATES & LIPIDS 11. Bioenergetics:: The Role of ATP 12. Biologic Oxidation 13. The Respiratory Chain & Oxidative Phosphorylation 14. Carbohydrates of Physiologic Significance 15. Lipids of Physiologic Significance 16. Overview of Metabolism & the Provision of Metabolic Fuels 17. The Citric Acid Cycle:: The Catabolism of Acetyl-CoA 18. Glycolysis & the Oxidation of Pyruvate 19. Metabolism of Glycogen 20. Gluconeogenesis & the Control of Blood Glucose 21. The Pentose Phosphate Pathway & Other Pathways of Hexose Metabolism 22. Oxidation of Fatty Acids:: Ketogenesis 23. Biosynthesis of Fatty Acids & Eicosanoids 24. Metabolism of Acylglycerols & Sphingolipids 25. Lipid Transport & Storage 26. Cholesterol Synthesis, Transport, & Excretion
SECTION III. METABOLISM OF PROTEINS & AMINO ACIDS 27. Biosynthesis of the Nutritionally Nonessential Amino Acids 28. Catabolism of Proteins & of Amino Acid Nitrogen 29. Catabolism of the Carbon Skeletons of Amino Acids 30. Conversion of Amino Acids to Specialized Products 31. Porphyrins & Bile Pigments
SECTION IV. STRUCTURE, FUNCTION, & REPLICATION OF INFORMATIONAL MACROMOLECULES 32. Nucleotides 33. Metabolism of Purine & Pyrimidine Nucleotides 34. Nucleic Acid Structure & Function 35. DNA Organization, Replication, & Repair 36. RNA Synthesis, Processing, & Modification 37. Protein Synthesis & the Genetic Code 38. Regulation of Gene Expression 39. Molecular Genetics, Recombinant DNA, & Genomic Technology
SECTION V. BIOCHEMISTRY OF EXTRACELLULAR & INTRACELLULAR COMMUNICATION 40. Membranes:: Structure & Function 41. The Diversity of the Endocrine System 42. Hormone Action & Signal Transduction
SECTION VI. SPECIAL TOPICS 43. Nutrition, Digestion, & Absorption 44. Micronutrients:: Vitamins & Minerals 45. Intracellular Traffic & Sorting of Proteins 46. Glycoproteins 47. The Extracellular Matrix 48. Muscle & the Cytoskeleton 49. Plasma Proteins & Immunoglobulins 50. Hemostasis & Thrombosis 51. Red & White Blood Cells 52. Metabolism of Xenobiotics