Despite extensive physiological, biochemical, and structural studies, the mechanisms of muscle contraction operating in living muscle fibres are still not well understood. This book aims to describe critically various experimental methods currently used in the field of muscle research. For each method discussed, there is a comprehensive description of its advantages, problems, and limitations. Each chapter also contains a summary of the central results to have been obtained usingeach method. Comprehensively written by experts in their respective fields, this book will be of interest to all investigators with an interest in muscle physiology.
Chapter 1 - Combined biochemical and mechanical experiments; Muscle mechanics I - intact single muscle fibres; Muscle mechanics II - skinned muscle fibres; The use of caged compounds; The use of fluorescent nucleotides; The use of peptide mimetics; The use of recombinant molecules; In vitro motility assay; Chapter 2 - Combined structural and mechanical experiments; The use of fluorescent probes; The use of spin probes; Time resolved X-ray diffraction; Electron microscopy coupled with quick freezing; Chapter 3 - The use of Ca2+ indicators; Chapter 4 - Electrophysiological techniques; Chapter 5 - Techniques for muscle energetics;
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