This practical guide covers all aspects of in situ hybridization, describing:: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take into account the many advances in the last seven years.
Principles of in situ hybridization; The design and generation of probes for in situ hybridization; The preparation of non-radioisotopic hybridization probes; Principles and applications of complementary RNA probes; Quantification of in situ hybridization; Quantification of grains in in situ hybridization; Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization; Single and simultaneous nucleic acid detection in clinical samples; Combined in situ hybridization and immunocytochemistry:: investigation of viral pathogenesis at the light and electron microscope levels; Super sensitive in situ DNA and RNA localisation;
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