This fully updated new edition of a successful and popular practical guide is an indispensable account of modern in-vitro fertilization practice. Initial chapters cover theoretical aspects of gametogenesis and embryo development at the cellular and molecular level, while the latter half of the book describes the requisites for a successful IVF laboratory and the basic technologies in ART. Advanced techniques, including pre-implantation genetic diagnosis, vitrification and stem-cell technology, are comprehensively covered, providing up-to-date analyses of these groundbreaking technologies. This edition includes:: ˘ New practical techniques, including preservation of fertility for cancer patients, stem-cell biology/technology, vitrification and in-vitro maturation ˘ A refresher study review of fundamental principles of cell and molecular biology ˘ The latest information available from animal and human research in reproductive biology Packed with a wealth of practical and scientific detail, this is a must for all IVF practitioners.
Preface; 1. Review of cell and molecular biology; 2. Endocrine control of reproduction:: Controlled Ovarian Hyperstimulation for ART; 3. Gametes and gametogenesis; 4. Sperm-oocyte interaction; 5. First stages of development; 6. Implantation and early stages of fetal development; 7. Stem cell biology; 8. The clinical in vitro fertilization laboratory; 9. Quality management in the IVF laboratory; 10. Sperm and ART; 11. Oocyte retrieval and embryo culture; 12. Cryopreservation of gametes and embryos; 13. Micromanipulation techniques; 14. Preimplantation genetic diagnosis Joyce Harper; 15. Epigenetics and assisted reproduction John Huntriss; Index.