This innovative book is one of the first resources to describe in detail the technique of digital time-lapse microscopy, a state-of-the-art analytical tool which is revolutionizing the field of assisted reproduction. Over 180 high-quality video sequences, accessible online via the password included in the book, provide a practical and highly visual guide to this new technology and the wealth of detail it can reveal about human embryo development. Written by a team of experts from across numerous clinical and scientific subspecialties, this book is a comprehensive guide to all aspects of the technique. It covers both the general principles of time-lapse microscopy and the specifics of working with various devices, with chapters on EmbryoScopeTM, Primo VisionTM and EevaTM as well as set-up and troubleshooting. Full electronic access to all text, images and supplementary videos makes this the ideal everyday reference for embryologists, clinicians and others working in IVF laboratories.
1. Introduction:: IVF concepts, embryo development and embryo selection Thomas Ebner; 2. Description of time-lapse systems:: EmbryoScopeTM Markus Montag, Tine Q. Kajhřj and Inge E. Agerholm; 3. Description of time-lapse systems:: Primo VisionTM Csaba Pribenszky; 4. Description of time-lapse systems:: the EevaTM test Lei Tan, Shehua Shen and Alice A. Chen; 5. Morphological cytoplasmic oocyte alterations:: embryo kinetics of dysmorphic oocytes Belén Aparicio-Ruiz and Maria Jose de los Santos; 6. Evaluating embryo development stages using time-lapse microscopy Thomas Freour; 7. Embryo quality (classification and selection) Alberto Tejera, Natalia Basile, Maura Caiazzo and Marcos Meseguer; 8. Time-lapse implementation in a clinical setting:: management of laboratory quality Dean E. Morbeck; 9. Time-lapse implementation in a clinical setting:: outcome results Nikica Zaninovic, Qiansheng Zhan and Zev Rosenwaks; 10. Unusual phenomena in embryonic development Giovanni Coticchio, Mariabeatrice Dal Canto, Mario Mignini Renzini and Rubens Fadini; Index.
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