This lab manual is designed for upper level undergraduates or graduate students, to introduce them to the field of developmental biology. After spending two weeks learning how to handle and manipulate a variety of embryonic organisms, students will begin a series of experiments that more or less keep pace with the sequence of most developmental biology textbooks (axial patterning, plant cell totipotency, fertilization, early plant development, morphogenesis, cell adhesion,embryogenesis, gametogenesis, regeneration and metamorphosis. The manual is heavily illustrated and gives students a solid grounding in classic developmental biology as well as modern techniques in immunohistochemistry and homeobox gene expression. Appendices of recipes, needed chemicals, and sources foranimals are included.
Table of Contents; The Experiments; Introduction to Developmental Biology: Embryo Protocols, Ethics and Model Systems; Axial Patterning: Using Retinoic Acid to Disrupt Homeobox Gene Expression in Axolotls; Plant Cell Totipotency: Growing a Carrot from Adult Cells; Fertilization: Sea Urchin Syngamy & Development; Early Plant Development: Pollen Tube Formation; Morphogenesis: Creating Fate Maps of Albino Axolotls Using a Vital Dye; Cell Adhesion: Cell-Cell Interactions in Sponges & Amphibians; Embryogenesis: Chick & Amphibian Development (Slide-based); Neurulation: Labelling Chick Notochord using Immunocytochemistry; Gametogenesis: Comparison of Plants and Animals; Regeneration: Morphallaxis & Epimorphosis; Metamorphosis: Drosphila Imaginal Discs; External Influences on Development: Design Your Own Experiment with Amphibians, Chicks or Fish; Information for the Instructor; Animal Care & Maintenance; Animal & Plant Sources; Chemical Sources; Slides Needed / Sources; Solutions & Other Materials; Glossary; Literature Cited;
Komentarze (0)
Chwilowo nie możesz polubić tej opinii
Zgłoś komentarz
Czy jesteś pewien, że chcesz zgłosić ten komentarz?
Zgłoszenie wysłane
Twój komentarz został wysłany i będzie widoczny po zatwierdzeniu przez moderatora.