Excited States and Free Radicals in Biology and Medicine

Excited states and free radicals are involved in the normal physiology of living systems; in pathological processes including lipid peoxidation, inflammation, Parkinsons disease, cancer, and ageing; in the mechanism of action of drugs, such as quinone antitumour agents, and in the photochemotherapies such as the PUVA therapy of skin diseases and PDT of cancer. The chief aim of this text is to introduce the reader to this rapidly expanding field, which lies at the borderlines ofphysics, chemistry, biology, pharmacology, and medicine, and, in particular, to explore how time-resolved spectroscopic methods have found solutions at the molecular level to biological and medical problems. In the first three chapters, the nature of excited states and free radicals, and the fast kinetic and spectroscopic techniques for their study are described. Amongst the biological areas described in the subsequent chapters are:: activated oxygen species; DNA; proteins; carotenoids and photosynthetic reaction centres; photodermatology and melanogenesis; and aspects of cancer related to radio-, chemo-, and photodynamic therapy.