Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry. The immune system of the body, in particular the role of antibodies, is described; and details provided on the isolation of antibody-producing cells, how hybridomas are formed and maintained in culture such that the required antibody can be produced to a very high degree of purity. The material is presented in such a way that students, researchers and managers in research and development will be able to make choices as to which are the most suitable techniques for their particular requirements. The text is comprehensively referenced and, in short, the book is a necessary resource for all those who have the need to be involved in the production of monoclonal antibodies from hybridoma cells in culture.
1. Introduction: the immune response; 2. Making hybridomas (hybridomas technology); 3. Factors affecting successful hybridoma production; 4. Selection of monoclonal antibody-secreting hybridoma cell lines; 5. The large-scale production of monoclonal antibodies in vitro; 6. In conclusion: the diversity in application of monoclonal antibodies and hybridoma technology; References; Index.
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